Compression of Histopathology, Culture and Rapid Urease Test in Diagnosis of Helicobacter Pylori in Gastric Biopsy Specimens
نویسندگان
چکیده مقاله:
Abstract Background and Objective: Helicobacter pylori (H. Pylori) infection is related to chronic gastritis, peptic ulcer, duodenal ulcer and gastric cancer. Thus, identification and treatment of the infection have a considerable importance. The aim of this study was to compare three methods of Histopathology, Culture and Rapid Urease test (RUT) in identification of H. Pylori in gastric biopsy specimens. Material and Methods: The participants were 153 patients (64 women and 89 men) suffering from digestive complaints, who referred to the endoscopy department of Shahid Beheshi Hospital in Hamadan, Iran. Three gastric biopsy samples were collected from each patient and examined by standard RUT, Histopathology and culture methods for diagnosis of H. Pylori. Results: Out of 153 patients, 69.9%, 27.4% and 2.6 % had gastritis, gastric ulcer and gastric cancer, respectively. The rate of infection with Urease test, culture and histology were identified 49.7%, 54.2%, and 89.5%, respectively. The sensitivity and specificity of the RUT result at first hour and after the first up to 24 hours were 55.4% and 80%, and 55.4% and 66/7%, respectively. The sensitivity and specificity of culture method were 60.6% and 100%, respectively. Conclusion: Based on the results, Histopathology method has a more sensitivity than both Culture method and rapid urease test for diagnosis of H. Pylori, and RUT is more specific when done in the first hour rather than after the first hour. Key words: Helicobacter pylori, rapid urease test methods, RUT, Histopathology
منابع مشابه
Comparison PCR Method and Rapid Urease Test to Detect Helicobacter Pylori in the Gastric Biopsy Tissue Samples
Background & Objective: Helicobacter pylori has been recognized as a major risk factor in gastric and duodenum ulcers and gastric cancer. Some laboratory tests, such as culture, are not entirely satisfying. The aim of this study is to compare RUT with PCR in identifying H. pylori in the gastric biopsy tissue samples.Materials & Methods: First, the standard H.pylori sample (N:oC30) was provided....
متن کاملInvestigation of H Pylori Colonization in Adenotonsillectomy Specimens by Means of Rapid Urease (CLO) Test
This prospective clinical study was performed to investigate the colonization of H pylori in adenoid and tonsil tissues by using the Campylobacter-like organism (CLO) test. Included in the study were 56 patients aged 3 to 43 yrs who had undergone adenoidectomy, tonsillectomy or adenotonsillectomy procedures under general anesthesia. Two-mm diameter tissue pieces of adenoid and tonsil specimens ...
متن کاملDetermination of Helicobacter pylori Antigen in Stool Samples and Comparison with Rapid Urease Test in Patients Suspected of Helicobacter Infection
Background and Aims: Helicobacter pylori (H. Pylori) is the microorganism that infects nearly half of word's population. There are several invasive and non-invasive methods for diagnosis of infection. The main objective of this study was to evaluate antigen of H. Pylori in feces with H. pylori stool antigen (HpSA) test and comparison with rapid urease test (RUT) in the patients suspected to be ...
متن کاملPCR and RFLP Analysis for Identification and Typing of Helicobacter pylori Strains from Gastric Biopsy Specimens
متن کامل
Polymerase chain reaction assay for the detection of Helicobacter pylori in gastric biopsy specimens: comparison with culture, rapid urease test, and histopathological tests.
Ulcer recurrence is probably related to residual Helicobacter pylori (H pylori). Histological examination and culture are considered to be the most specific tests. CLO test is a rapid but less specific test, which is usually used as an alternative test to culture. The aim of this study was to investigate the efficiency of a simplified polymerase chain reaction (PCR) assay as a procedure for the...
متن کاملRole of rapid urease test and histopathology in the diagnosis of Helicobacter pylori infection in a developing country
BACKGROUND The aim of this study was to determine the effect of commonly self-prescribed proton pump inhibitors (PPI) on the results of rapid urease test and histology for the diagnosis of H. pylori infection. METHODS One hundred-nine consecutive patients with dyspeptic symptoms attending the endoscopy suite were enrolled in this study. Antrum biopsy specimens were collected at endoscopy for ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 9 شماره 1
صفحات 32- 38
تاریخ انتشار 2015-04
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
کلمات کلیدی برای این مقاله ارائه نشده است
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023